
Product Name: Acid Red 52 / Sulforhodamine B
Color Index: C.I. 45100
CAS: 3520-42-1
Molecular Formula:C27H29N2NaO7S2
Formula Weight: 580.65 g/mol
Synonyms: Sulforhodamine B; Xylene Red B; Acid Rhodamine B; Lissamine Rhodamine; C.I. Acid Red 52; Brilliant Acid Rhodamine B; Food Red 106; Sanolin Rhodamine B; Water Red 27,Acid Red 3B; Acid Rose B; Acid Rose E-XB; Acid Peach B;
Application:
Textiles (Wool, Silk, Nylon), Cosmetics & Personal Care, Biological Staining & Research, Inkjet Inks, Stationery Products, Detergents & Cleaners, Fluorescent Pigments
1.Product Description
Experience the Brilliant Fluorescence of Acid Red 52 – The Premier Xanthene Dye for Science and Industry.
Acid Red 52, universally recognized as Sulforhodamine B, represents the gold standard among xanthene-based acid dyes, distinguished by its intense pink-red coloration and characteristic orange-yellow fluorescence . This sophisticated molecule features a central xanthene core with diethylamino groups that confer exceptional tinctorial strength, while multiple sulfonate groups ensure outstanding water solubility and anionic character .
What truly elevates Acid Red 52 above conventional acid dyes is its dual personality as both an industrial colorant and a scientific tool. In the dyehouse, it delivers brilliant bluish-pink shades on wool, silk, and nylon with excellent leveling properties and a distinctive fluorescent quality that adds visual depth to finished goods . The dye exhibits remarkable stability, maintaining its vibrant color across a wide pH range from 1 to 14, with optimal performance in neutral to slightly alkaline conditions .
In the laboratory, Sulforhodamine B has achieved iconic status through the SRB (Sulforhodamine B) cytotoxicity assay, a standard method endorsed by the National Cancer Institute for in vitro drug screening . The dye binds stoichiometrically to cellular proteins, enabling precise quantification of cell mass and proliferation—a testament to its reliability and reproducibility in biomedical research .
For advanced applications, Acid Red 52 serves as a photocatalyst under yellow light irradiation, facilitating acetalization reactions with yields up to 93%, and its fluorescence properties make it invaluable for DNA and RNA aptamer research . Our product is manufactured under strict quality controls to ensure consistent spectroscopic performance, low salt content, and batch-to-batch reproducibility across all grades.
2.Product Specifications (Technical Data Sheet)
| Property | Specification | Insight |
| Product Name | Acid Red 52 (Sulforhodamine B / Xylene Red B) | Premium xanthene dye for high-performance applications. |
| Appearance | Red to Dark Brown to Greenish-Brown Powder; may appear as dark blue to black powder/crystals | Color varies by concentration and crystal form. |
| CAS No. | 3520-42-1 | Verified chemical registry number . |
| EINECS/EC No. | 222-529-8 | Listed in European inventory . |
| Solubility | Water: 30-100 g/L (soluble); Ethanol: <10 g/L; DMSO: soluble | Excellent water solubility; forms clear, fluorescent pink solutions. |
| Solubility in Methanol | 1 mg/mL | Suitable for specialized formulations. |
| Style | Anionic / Xanthene Acid Dye | Suitable for application across wide pH range. |
| Tinting Strength | Absorbance E1%1cm ≥ 1,800 (564-568 nm) | High extinction coefficient ensures brilliant coloration. |
| λmax | 554-568 nm (in water/buffer) | Characteristic absorption peak for quality verification. |
| Extinction Coefficient | ≥ 85,000 at 554-560 nm (in 0.1M NaOH/methanol) | Exceptional molar absorptivity. |
| Purity (Dye Content) | Technical Grade: ≥75-80% / High Purity: ≥98% (HPLC) | Multiple purity grades available for diverse applications. |
| pH Stability Range | 1-14 | Exceptional stability across entire pH spectrum. |
| Optimal pH Range | 6-11 | Peak performance in neutral to alkaline conditions. |
| Packing | 25 kg HDPE bags / 500 kg jumbo bags / 10g-1kg research packs | Secure, moisture-proof packaging options available. |
| Melting Point | >300°C (Decomposes) | High thermal stability for industrial processing. |
| Storage Conditions | Room temperature in cool, dark, dry place | Stable for extended periods under proper storage. |
| Application | Textiles, Cosmetics, Biological Staining, Inks, Detergents, Research | Versatile across multiple industries. |
3.Product Application (Industry Usage Guide)
Textile Industry (Wool, Silk & Nylon):
Acid Red 52 is specifically designed for exhaust dyeing and direct printing of protein and polyamide fibers . Apply in weakly acidic to neutral baths (pH 5-7) for optimal exhaustion . Its unique properties include:
Fluorescent Effect: Imparts a distinctive orange-yellow fluorescence to dyed materials, creating visually striking effects .
Brilliant Shades: Produces clean bluish-pink to magenta colors with exceptional brightness .
Level Dyeing: Excellent migration properties ensure uniform coloration on challenging substrates.
Leather Dyeing: Effective for drum dyeing and spray applications on finished leathers .
Cosmetics & Personal Care:
As CI 45100, Acid Red 52 meets EU requirements for cosmetic ingredients and is widely used in :
Hair Colorants: Provides vibrant pink-red shades with good stability.
Bath & Shower Products: Stable in surfactant systems across pH 1-13 .
Soaps & Detergents: Excellent compatibility with household and I&I cleaning formulations .
Cosmetic Formulations: Used in lipsticks, blushes, and color cosmetics requiring bright pink hues.
Peroxide & Hypochlorite Stable: Maintains color integrity in bleach-containing products .
Biological Staining & Research:
Acid Red 52 is indispensable in life sciences :
SRB Cytotoxicity Assay: The gold standard for in vitro drug screening and cell proliferation studies, endorsed by the National Cancer Institute. The dye binds stoichiometrically to cellular proteins, enabling precise quantification of cell mass .
Histological Staining: Used in hematology and histology for tissue section visualization .
Microbial Identification: Suitable for staining applications in microbiology .
DNA/RNA Aptamer Research: Isolates fluorophore-specific aptamers for molecular recognition studies .
Photocatalysis: Acts as a photocatalyst under yellow light for organic synthesis .
Inks & Stationery:
Acid Red 52 is a preferred colorant for :
Inkjet Inks: Provides brilliant magenta shades with excellent jetting properties .
Sign Pens & Roller Pens: Consistent flow and vibrant color for writing instruments .
Fountain Pens & Stamp Pads: High solubility prevents clogging and ensures smooth application .
Low Salt Formulations: Available in low-salt grades for demanding ink applications .
Detergents & Cleaners:
The dye is highly recommended for :
Laundry Detergents & Fabric Softeners: Provides aesthetic coloration to liquid products.
Dishwashing Detergents: Stable in alkaline formulations.
Industrial & Institutional Cleaners: Compatible with a wide range of cleaning chemistries.
pH Versatility: Maintains stability from pH 1-13, making it suitable for both acidic and alkaline cleaners .
Fluorescent Pigments:
Acid Red 52 is used in the manufacture of daylight fluorescent pink pigments for specialty coatings and plastics.
4.Packaging
Usually is 25Kg/drum or paper box or bag with inner double PE liner Or provide customized packaging according to customer requirements.
5.Our advantage
1) Provide free samples for testing or comparing the effect difference and cost performance.
2) Fast response speed, customers' questions at any time will be responded to in two hours.
3) Fast delivery: the fastest delivery can be made on the same day.
4)Different packaging solutions: provide various packaging options according to customer requirements.
5)Technical guidance: provide improved whitening and coloring formulas and guidance according to customer production materials.
6) Sample retention or sample mailing before bulk delivery.
7) Providing certificationes, such as Reach certification.
8) Different payment methods: TT LC, etc.
9) Providing door-to-door DDP service.